Bacterial Transformation (LabXChange) Simulation Worksheet
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Use this worksheet to help your students through TWO online lab simulations on LabXChange's website. Use chemically competent cells and heat shock to transform bacteria in the first lab of the document. Take those same transformed bacteria into the second lab to plate them on solid agar and evaluate the growth and success of your experiment.
Pairs perfectly with the "Digest, Ligate, and Evaluate Plasmids (LabXChange) Simulation Worksheet"
**Note: depending on your class times, these activities can take 3 days to complete. A great option for a lab practical!
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Modeling Bacteria Transformation Worksheet
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Biology Teaching Resources
Case Study: How Do Bacteria Become Resistant?
Students analyze data regarding methicillin and penicillin resistant bacteria, focusing how hospitals inadvertently drive the evolution of resistant strains. Each section of the case explores an aspect of dealing with bacteria, including sanitation, analyzing agar plates uses the Kirby-Bauer disk method and concludes with students suggesting plans for reducing the incidence of MRSA in hospitals.
With bacteria resistance becoming more common, traditional classrooms may be reluctant to do labs involving bacteria cultures, so this activity can be a good substitute. It involves data analysis and examination of images that show how disks soaked in antibiotics would affect growth.
I still grow bacteria on pre-poured agar plates , but no longer allow students to manipulate or take samples from unknown colonies. There are disposed of in buckets of bleach.
Older students, like my AP Biology class, still do the bacteria transformation lab , but these are older, college track students and the bacteria are grown from known samples of “harmless” strains. For them, this is a good experience for learning sterile technique.
Grade Level: 9-12 | Time: 1 – 1.5 hours
HS-LS4-3 – Apply concepts of statistics and probability to support explanations that organisms with an advantageous heritable trait tend to increase in proportion to organisms lacking this trait. HS-LS4-4 – Construct an explanation based on evidence for how natural selection leads to adaptation of populations.
Shannan Muskopf
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Photosynthesis Case Study 2021-2; Case Study - Driving is Dangerous ... UNV-100-Compare Contrast Pre-Writing Chart; Preview text. Bacterial Transformation pre-LAB and study guide: Name three genes the pGLO plasmid contains: How does araC regulate GFP? Complete the basic procedure: ... Artificial selection worksheet. AP Biology 100% (1) 1 ...
Pre-Lab Student Worksheet. Watch the "Avery's Transforming Principle" animation on the following webpage, and answer the questions that follow. 1. Describe the difference between the two strains of bacteria used in Griffith's experiments. 2. Which of the two bacterial strains in Griffith's experiments has the ability to cause ...
Bacteria Transformation Activity—Modeling Bacteria Transformation Worksheet Answer Key Modeling Bacteria Transformation Worksheet Answer Key DATA and OBSERVATIONS [ Staple recombinant model here. ] OVERVIEW of BACTERIA TRANSFORMATION Instructions: Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria
In this investigation, students will first acquire the tools to transform E. coli bacteria to express new genetic information using a plasmid system and apply mathematical routines to determine transformation efficiency. (Competent bacterial cells are able to take up exogenous genetic material and are capable of being transformed, and the
Study with Quizlet and memorize flashcards containing terms like plasmid, genome, DNA and more. ... bacteria transformation worksheet. Flashcards; Learn; Test; Match; Q-Chat; Get a hint. plasmid. circular DNA found in bacteria. 1 / 7. 1 / 7. ... bacteria simple structure no membrane bound organelles.
1. What is the name of the scientist who published a study in 1928 on 2 different strains of Pneumococcus bacteria? 2. In the animation there are images of smooth (S) bacteria and rough (R) bacteria. These different types of bacteria are named after their different physical traits. Explain how differences in DNA can result in different traits. 3.
This bundle includes 3 worksheets that correspond to SIX LabXChange labs (that are not flash based). These five labs cover restriction digests, ligations, electrophoresis evaluation of plasmids, bacterial transformation, and plating transformed bacteria using antibiotic selective measures. **Bonus: 3. Products. $4.50 $5.50 Save $1.00. View Bundle.
Terms in this set (20) Bacterial Transformation. process by which bacterial cells take up naked DNA molecules, and such DNA will be replicated by the bacteria along its own DNA, if the foreign DNA has an origin of replication recognized by the host cell DNA polymerases. competent bacteria. bacteria which are able to uptake DNA.
This quiz/worksheet combo will allow students to test the following skills: Making connections - use understanding of the concept of bacterial transformation. Defining key concepts - ensure that ...
Procedure. -Add CaCl2 (calcium chloride) *Causes the cell to swell with influx of water and chloride ions, necessary for the update of DNA. *Promotes binding of plasmid DNA to E. Coli cell wall. -Heat Shock. *So bacteria can take in the plasmid at 42 degrees C, at set of genes are expressed which help the bacteria in surviving at this temperature.
Modeling Bacteria Transformation Worksheet Answer Key. DATA and OBSERVATIONS. [ Staple recombinant model here. OVERVIEW of BACTERIA TRANSFORMATION. Instructions: Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria. transformation and the lettered lines with the name of the structure next to it.
Bacteria Transformation Activity—Modeling Bacteria Transformation Worksheet Modeling Bacteria Transformation Worksheet DATA and OBSERVATIONS [ Staple recombinant model here. ] OVERVIEW of BACTERIA TRANSFORMATION Instructions: Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria
Worksheet. Print Worksheet. 1. Biotech companies have exploited transformation in order to _____. insert and express genes in competent bacteria. increase the rate of cell division. force non ...
About This Quiz & Worksheet. Use this worksheet and quiz to quantify your knowledge of a bacterial transformation biology lab. Concepts you will need to know include identifying a word to describe ...
- Data collection worksheet for fundamental microbiology LAB. redesigning bacteria data collection worksheet responses must be in your own words and in complete ... BIO 211L Exam 2 Study Guide. FUNDAMENTALS MICROBIOLOGY ... therefore if results indicate growth this could mean the potential transformation of the bacteria inoculated. Look closely ...
With bacteria resistance becoming more common, traditional classrooms may be reluctant to do labs involving bacteria cultures, so this activity can be a good substitute. It involves data analysis and examination of images that show how disks soaked in antibiotics would affect growth. I still grow bacteria on pre-poured agar plates, but no ...
The null hypothesis is that the E. Coli bacteria will glow and grow the most under +pGLO with LB/Ara. While the alternative hypothesis is that the E. Coli bacteria will glow, and grow the most under +pGLO with LB/Ara/Amp. Bacterial transformation is crucial for in science because it allows genetically engineered bacteria to produce medicines.